Here are five key points from the boss ahead of the trip to St Marys.
Both Mohamed Salah and Virgil van Dijk limped out of Saturdays FA Cup final triumph over Chelsea, leading to immediate concern surrounding their involvement in the Champions League final.
Neither are likely to start on Tuesday night, with a more realistic target being the visit of Wolves on Sunday, and Klopp expects to make a lot of decisions over the rest of his lineup.
When the boys arrive today, I have to make a lot of decisions, but we will line up a team to try to win at Southampton, he said.
That is our job. The 30 extra minutes [on Saturday] made a big difference. We have to see.
Meanwhile, the German encouragingly added that Fabinho is doing very well in his own injury recovery, but wont be available at St Marys.
Man Citys 2-2 draw away to West Ham has given Liverpool hope in the title race, with victory on Tuesday taking them to within one point ahead of the final day.
That would then lead to the Steven Gerrard narrative taking over for the week, as the Reds legend looks to do his boyhood club the biggest favour imaginable on Sunday.
Klopp admitted that he wasnt expecting City to drop points at home to Villa, however, especially given their ability to bounce back from poor results.
I dont know when the last time they dropped points twice in a week was, historically, Klopp added.
I dont expect City to drop points, but in an ideal world, we go into Sunday one point behind.
Liverpools players celebrated their FA Cup win wildly, having been handed their medals after the game something that they will treasure for the rest of their lives.
Unfortunately, in true FA fashion, there were only so many to go around, meaning some of Klopps squad werent given one.
Dear FA please send us some more medals! I dont know why the number is restricted, Klopp half-joked.
How do they not think these things through? Its extraordinary.
A huge amount was made of Liverpools fans booing the national anthem at Wembley wouldnt it be nice if the media got angrier about more important things?
Klopp gave a typically measured answer when he was quizzed about his thoughts on the situation, sticking up for his supporters:
Our people wouldnt do it if there was no reason. It is something historical.
The majority of our support are wonderful people. Really smart, go through lows and highs. They wouldnt do it without reason.
The man never misses a step.
It could be a fatigued Liverpool who take to the field at St Marys, given the physical exertions of the squad at Wembley.
Klopp has voiced his frustration about the scheduling of games in the past and the quick turnaround for the Southampton game has clearly irked him.
We could have played Wednesday but cant because of the Europa League final, right? Ahhh, UEFA, my friends! he said.
We could have played Thursday. I have to sit here and say this. Nobody thinks about it apart from me 120 minutes on Saturday, then we play Tuesday. Come on.